A1978
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 | Immunoblotting Chicken fibroblast cells were separated on SDS-PAGE and probed with Monoclonal Anti-β-Actin, clone AC-15 (Cat. No. A1978). The antibody was developed with Anti-Mouse IgG (Fab), Alkaline Phosphatase (Cat. No. A1293) and a NBT/BCIP substrate. Antibody concentration 0.5 μg/mL. |
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A1978
Sigma
Monoclonal Anti-β-Actin antibody produced in mouse
clone AC-15, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-β-Actin |
Description
| Application | The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin's solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding. |
| Immunogen | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | AC-15 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine |
| does not react with | Dictyostelium discoideum |
| application(s) | immunoblotting: 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts. |
| immunocytochemistry: 10-40 μg/mL using human foreskin fibroblasts | |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... ACTB(60) |
| rat ... Actb(81822) | |
| mouse ... Actb(11461) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | North, A.J., et al. J. Cell Sci. 107, 445-455, (1994) Abstract |
| Gimona, M., et al. Cell Motil., 108-116, (1994) | |
| Roizman, B., et al., The Interaction of Herpes Simplex Virus 1 Regulatory Protein ICP22 with the cdc25C Phosphatase Is Enabled in vitro by Viral Protein Kinases US3 and UL13 J. Virol. 82, 4533-4543, (2008) Abstract | |
| Lowe, D.A., et al., Skeletal muscle-specific ablation of cyto-actin does not exacerbate the mdx phenotype. PLoS ONE 3, e2419, (2008) Abstract | |
| Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994) | |
| North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994) | |
| North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994) | |
| Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000) |
